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PMID: 19781752 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Condensins regulate meiotic DNA break distribution, thus crossover frequency, by controlling chromosome structure.

Cell ·Vol. 139 ·No. 1 ·2009-10-02 ·Pages 73-86

Mets DG, Meyer BJ

Abstract

Meiotic crossover (CO) recombination facilitates evolution and accurate chromosome segregation. CO distribution is tightly regulated: homolog pairs receive at least one CO, CO spacing is nonrandom, and COs occur preferentially in short genomic intervals called hotspots. We show that CO number and distribution are controlled on a chromosome-wide basis at the level of DNA double-strand break (DSB) formation by a condensin complex composed of subunits from two known condensins: the C. elegans dosage compensation complex and mitotic condensin II. Disruption of any subunit of the CO-controlling condensin dominantly changes DSB distribution, and thereby COs, and extends meiotic chromosome axes. These phenotypes are cosuppressed by disruption of a chromosome axis element. Our data implicate higher-order chromosome structure in the regulation of CO recombination, provide a model for the rapid evolution of CO hotspots, and show that reshuffling of interchangeable molecular parts can create independent machines with similar architectures but distinct biological functions.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Animals Caenorhabditis elegans/genetics,metabolism Caenorhabditis elegans Proteins/genetics,metabolism Chromosomal Proteins, Non-Histone Crossing Over, Genetic DNA Breaks, Double-Stranded DNA-Binding Proteins/genetics,metabolism Humans Meiosis Multiprotein Complexes/genetics,metabolism Mutation Rad51 Recombinase/metabolism
Chemicals
Caenorhabditis elegans Proteins Chromosomal Proteins, Non-Histone DNA-Binding Proteins Dpy-28 protein, C elegans HIM-3 protein, C elegans Multiprotein Complexes condensin complexes Rad51 Recombinase rad-51 protein, C elegans Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mets David G
Howard Hughes Medical Institute, University of California-Berkeley, Berkeley, CA 94720-3204, USA.
Meyer Barbara J
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2009.07.035
Published
2009-10-02
Epub
2009-00-24
Pages
73-86
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2785808
Subset
IM
Grants
NIGMS NIH HHS · R01 GM030702 · United States
NIGMS NIH HHS · T32 GM 07232 · United States
NIGMS NIH HHS · T32 GM007232 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · T32 GM 07127 · United States
NIGMS NIH HHS · R01 GM030702-28 · United States
NIGMS NIH HHS · T32 GM007127 · United States
Corrections
CommentIn
PMID: 19804748
External Links
PubMed source DOI: 10.1016/j.cell.2009.07.035 PMC Full Text View journal details
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