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PMID: 2247072 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6544-53

All-Robyn JA, Brown N, Otaka E, Liebman SW

Abstract

The accurate and efficient translation of proteins is of fundamental importance to both bacteria and higher organisms. Most of our knowledge about the control of translational fidelity comes from studies of Escherichia coli. In particular, ram (ribosomal ambiguity) mutations in structural genes of E. coli ribosomal proteins S4 and S5 have been shown to increase translational error frequencies. We describe the first sequence of a ribosomal protein gene that affects translational ambiguity in a eucaryote. We show that the yeast omnipotent suppressor SUP44 encodes the yeast ribosomal protein S4. The gene exists as a single copy without an intron. The SUP44 protein is 26% identical (54% similar) to the well-characterized E. coli S5 ram protein. SUP44 is also 59% identical (78% similar) to mouse protein LLrep3, whose function was previously unknown (D.L. Heller, K.M. Gianda, and L. Leinwand, Mol. Cell. Biol. 8:2797-2803, 1988). The SUP44 suppressor mutation occurs near a region of the protein that corresponds to the known positions of alterations in E. coli S5 ram mutations. This is the first ribosomal protein whose function and sequence have been shown to be conserved between procaryotes and eucaryotes.

Related Genes
SUP44 ram
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Genes, Fungal Genes, Suppressor Genetic Complementation Test Molecular Sequence Data Mutation Restriction Mapping Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid
Chemicals
Ribosomal Proteins ribosomal protein S4
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
All-Robyn J A
Department of Biological Sciences, University of Illinois, Chicago 60680.
Brown N
Otaka E
Liebman S W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6544-6553.1990
Published
1990-12-00
Pages
6544-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362931
Subset
IM
Grants
NIGMS NIH HHS · GM-24189 · United States
Databases
GENBANK
M38029, M59375
External Links
PubMed source DOI: 10.1128/mcb.10.12.6544-6553.1990 PMC Full Text View journal details
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