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PMID: 2784204 Published · ppublish English Journal Article

Cloning and structure of the BepI modification methylase.

Nucleic acids research ·Vol. 17 ·No. 3 ·1989-02-11 ·Pages 1077-88

Kupper D, Zhou JG, Venetianer P, Kiss A

Abstract

The gene coding for a CGCG specific DNA methylase has been cloned in E. coli from Brevibacterium epidermidis. The enzyme, named BepI methylase, is probably the cognate methylase of the FnuDII isoschizomer BepI endonuclease isolated from this strain. The expression of BepI methylase in E. coli is dependent on the orientation of the cloned fragment suggesting that the gene is transcribed from a promoter on the plasmid vector. No BepI endonuclease could be detected in the clones producing BepI methylase. The nucleotide sequence of the BepI methylase gene has been determined, it predicts a protein of 403 amino acids (MR: 45,447). Analysis of the amino acid sequence deduced from the nucleotide sequence revealed similarities between the BepI methylase and other cytosine methylases. M. BepI methylates the external cytosine in its recognition sequence.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Base Sequence Brevibacterium/enzymology,genetics Cloning, Molecular DNA-Cytosine Methylases/genetics,isolation & purification Deoxyribonucleases, Type II Site-Specific/genetics,isolation & purification Molecular Sequence Data Sequence Homology, Nucleic Acid Structure-Activity Relationship
Chemicals
Bacterial Proteins DNA modification methylase BsuE DNA-Cytosine Methylases CGCG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kupper D
Biological Research Center, Hungarian Academy of Sciences, Szeged.
Zhou J G
Venetianer P
Kiss A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.3.1077
Published
1989-02-11
Pages
1077-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331723
Subset
IM
Databases
GENBANK
X13555
External Links
PubMed source DOI: 10.1093/nar/17.3.1077 PMC Full Text View journal details
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