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PMID: 2989774 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Presence of a limited number of essential nucleotides in the promoter region of mouse ribosomal RNA gene.

Nucleic acids research ·Vol. 13 ·No. 10 ·1985-05-24 ·Pages 3515-32

Kishimoto T, Nagamine M, Sasaki T, Takakusa N, Miwa T, Kominami R, Muramatsu M

Abstract

Point mutations are introduced into a mouse rDNA fragment containing the promoter region by a sodium bisulfite method and the mutants are tested for the ability of accurate transcription initiation in vitro. The results indicate that the change, G to A, at -7 completely eliminates the promoter activity, and those at -16 and at -25 decrease it to about 10% and 50%, respectively. On the other hand, the substitutions at +9, +4, -2, -9 and -39 do not alter the template activity significantly. It is concluded that there are limited but distinct nucleotides that are essential for the transcription initiation of this gene. This sort of absolute requirement for single specific bases is not reported in protein coding genes transcribed by RNA polymerase II. We propose that these rigid recognition signals which we have found are the molecular basis for the strong species-dependency of the transcription machinery of RNA polymerase I system. A model is presented in which a transcription factor interacts with the rDNA promoter from one side of the DNA double-helix with essential contacts at these bases.

MeSH Terms
Animals Base Composition DNA, Ribosomal/analysis Endonucleases/pharmacology Mice Mutation Nucleotides/analysis Promoter Regions, Genetic RNA Polymerase I/pharmacology RNA, Ribosomal/analysis Single-Strand Specific DNA and RNA Endonucleases Species Specificity Transcription, Genetic
Chemicals
DNA, Ribosomal Nucleotides RNA, Ribosomal RNA Polymerase I Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kishimoto T
Nagamine M
Sasaki T
Takakusa N
Miwa T
Kominami R
Muramatsu M
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.10.3515
Published
1985-05-24
Pages
3515-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341256
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.10.3515 PMC Full Text View journal details
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