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PMID: 30909785 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Identification of transcription factors that regulate ATG8 expression and autophagy in Arabidopsis.

Autophagy ·Vol. 16 ·No. 1 ·2020-00-00 ·Pages 123-139

Wang P, Nolan TM, Yin Y, Bassham DC

Abstract

Autophagy is a conserved catabolic process in eukaryotes that contributes to cell survival in response to multiple stresses and is important for organism fitness. In Arabidopsis thaliana, the core machinery of autophagy is well defined, but its transcriptional regulation is largely unknown. The ATG8 (autophagy-related 8) protein plays central roles in decorating autophagosomes and binding to specific cargo receptors to recruit cargo to autophagosomes. We propose that the transcriptional control of ATG8 genes is important during the formation of autophagosomes and therefore contributes to survival during stress. Here, we describe a yeast one-hybrid (Y1H) screen for transcription factors (TFs) that regulate ATG8 gene expression in Arabidopsis, using the promoters of 4 ATG8 genes. We identified a total of 225 TFs from 35 families that bind these promoters. The TF-ATG8 promoter interactions revealed a wide array of diverse TF families for different promoters, as well as enrichment for families of TFs that bound to specific fragments. These TFs are not only involved in plant developmental processes but also in the response to environmental stresses. TGA9 (TGACG (TGA) motif-binding protein 9)/AT1G08320 was confirmed as a positive regulator of autophagy. TGA9 overexpression activated autophagy under both control and stress conditions and transcriptionally up-regulated expression of ATG8B, ATG8E and additional ATG genes via binding to their promoters. Our results provide a comprehensive resource of TFs that regulate ATG8 gene expression and lay a foundation for understanding the transcriptional regulation of plant autophagy.Abbreviations: ABRC: Arabidopsis biological resource center; AP2-EREBP: APETALA2/Ethylene-responsive element binding protein; ARF: auxin response factor; ATF4: activating transcription factor 4; ATG: autophagy-related; ChIP: chromatin immunoprecipitation; DAP-seq: DNA affinity purification sequencing; FOXO: forkhead box O; GFP: green fluorescent protein; GO: gene ontologies; HB: homeobox; LD: long-day; LUC: firefly luciferase; MAP1LC3: microtubule associated protein 1 light chain 3; MDC: monodansylcadaverine; 3-MA: 3-methyladenine; OE: overexpressing; PCD: programmed cell death; qPCR: quantitative polymerase chain reaction; REN: renilla luciferase; RT: room temperature; SD: standard deviation; TF: transcription factor; TFEB: transcription factor EB; TGA: TGACG motif; TOR: target of rapamycin; TSS: transcription start site; WT: wild-type; Y1H: yeast one-hybrid.

Keywords
ATG8 promoters Arabidopsis thaliana TGA9 autophagy transcription factors yeast one-hybrid screen
MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/metabolism Autophagosomes/metabolism Autophagy/physiology Autophagy-Related Protein 8 Family/metabolism Carrier Proteins/metabolism DNA-Binding Proteins Plant Proteins Promoter Regions, Genetic/genetics Stress, Physiological/physiology Transcription Factors/metabolism
Chemicals
ATG8 protein, Arabidopsis Arabidopsis Proteins Autophagy-Related Protein 8 Family Carrier Proteins DNA-Binding Proteins Plant Proteins Transcription Factors ethylene-responsive element binding protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang Ping
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA. | State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Nolan Trevor M
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
Yin Yanhai
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
Bassham Diane C ORCID
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
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Article Info
Journal
Autophagy
Abbr.
Autophagy
ISSN
1554-8635
DOI
10.1080/15548627.2019.1598753
Published
2020-00-00
Epub
2019-00-06
Pages
123-139
Language
English
Region
United States
NLM ID
101265188
PMCID
PMC6984607
Subset
IM
Grants
NIGMS NIH HHS · R01 GM120316 · United States
External Links
PubMed source DOI: 10.1080/15548627.2019.1598753 PMC Full Text View journal details
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