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PMID: 3665882 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A hotspot for novel amplification joints in a mosaic of Alu-like repeats and palindromic A + T-rich DNA.

The EMBO journal ·Vol. 6 ·No. 8 ·1987-08-00 ·Pages 2401-8

Hyrien O, Debatisse M, Buttin G, de Saint Vincent BR

Abstract

We have identified, in the amplified domain of adenylate deaminase (AMPD) overproducing Chinese hamster fibroblasts, a 2.6 kb recombinogenic DNA region which is frequently involved in amplification-associated rearrangements. The nucleotide sequence reveals a mosaic organization of four Alu-equivalent repeats of the B1 and B2 families and eight long A + T-rich DNA segments. Part of this region is enriched with long imperfect palindromes. The center of one palindrome contains a putative topoisomerase I cleavage site and this site defines the position of a novel junction which was formed by illegitimate recombination with anther A + T-rich DNA sequence located far apart on the amplified DNA. These findings and their significance are discussed in the context of related data from other systems and in the light of current models for eukaryotic DNA recombination, replication and organization.

MeSH Terms
AMP Deaminase/genetics Adenine Animals Base Sequence Cell Line Cloning, Molecular Gene Amplification Genes Nucleic Acid Conformation Nucleotide Deaminases/genetics Repetitive Sequences, Nucleic Acid Thymine
Chemicals
Nucleotide Deaminases AMP Deaminase Adenine Thymine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hyrien O
Unité de Génétique Somatique (UA CNRS 361), Institut Pasteur, Paris, France.
Debatisse M
Buttin G
de Saint Vincent B R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-08-00
Pages
2401-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553646
Subset
IM
Databases
GENBANK
X05583, X05584, Y00077
External Links
PubMed source PMC Full Text View journal details
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