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PMID: 7638211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conditional expression of the ubiquitous transcription factor MafK induces erythroleukemia cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 16 ·1995-08-01 ·Pages 7445-9

Igarashi K, Itoh K, Hayashi N, Nishizawa M, Yamamoto M

Abstract

Transcription factor NF-E2 activity is thought to be crucial for the transcriptional regulation of many erythroid-specific genes. The three small Maf family proteins (MafF, MafG, and MafK) that are closely related to the c-Maf protooncoprotein constitute half of the NF-E2 activity by forming heterodimers with the large tissue-restricted subunit of NF-E2 called p45. We have established and characterized murine erythroleukemia cells that conditionally overexpress MafK from a metallothionein promoter. The conditional expression of MafK caused accumulation of hemoglobin, an indication of terminal differentiation along the erythroid pathway. Concomitantly, DNA binding activities containing MafK were induced within the MafK-overexpressing cells. These results demonstrate that MafK can promote the erythroid differentiation program in erythroleukemia cells and suggest that the small Maf family proteins are key regulatory molecules for erythroid differentiation.

Related Genes
mafK
MeSH Terms
Animals Base Sequence Cell Differentiation/genetics Cell Line, Transformed DNA Probes/genetics DNA, Neoplasm/genetics,metabolism Erythropoiesis/genetics Gene Expression Humans Leukemia, Erythroblastic, Acute/genetics,metabolism,pathology MafK Transcription Factor Mice Molecular Sequence Data Nuclear Proteins/genetics,metabolism Protein Binding Tumor Cells, Cultured
Chemicals
DNA Probes DNA, Neoplasm MafK Transcription Factor Nuclear Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Igarashi K
Department of Biochemistry, Tohoku University School of Medicine, Sendai, Japan.
Itoh K
Hayashi N
Nishizawa M
Yamamoto M
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.16.7445
Published
1995-08-01
Pages
7445-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41356
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.16.7445 PMC Full Text View journal details
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