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PMID: 7891713 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.

Molecular and cellular biology ·Vol. 15 ·No. 4 ·1995-04-00 ·Pages 2180-90

Kataoka K, Igarashi K, Itoh K, Fujiwara KT, Noda M, Yamamoto M, Nishizawa M

Abstract

The maf oncogene encodes a bZip nuclear protein which recognizes sequences related to an AP-1 site either as a homodimer or as heterodimers with Fos and Jun. We describe here a novel maf-related gene, mafG, which shows extensive homology with two other maf-related genes, mafK and mafF. These three maf-related genes encode small basic-leucine zipper proteins lacking the trans-activator domain of v-Maf. Bacterially expressed small Maf proteins bind to DNA as homodimers with a sequence recognition profile that is virtually identical to that of v-Maf. As we have previously described, the three small Maf proteins also dimerize with the large subunit of NF-E2 (p45) to form an erythroid cell-specific transcription factor, NF-E2, which has distinct DNA-binding specificity. This study shows that the small Maf proteins can also dimerize among themselves and with Fos and a newly identified p45-related molecule (Ech) but not with v-Maf or Jun. Although the small Maf proteins preferentially recognize the consensus NF-E2 sequence as heterodimers with either NF-E2 p45, Ech, or Fos, these heterodimers seemed to be different in their transactivation potentials. Coexpression of Fos and small Mafs could not activate a promoter with tandem repeats of the NF-E2 site. These results raise the possibility that tissue-specific gene expression and differentiation of erythroid cells are regulated by competition among Fos, NF-E2 p45, and Ech for small Maf proteins and for binding sites.

Related Genes
fos jun mafG
MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens Consensus Sequence DNA-Binding Proteins/genetics,metabolism Erythroid-Specific DNA-Binding Factors Gene Expression Regulation Genomic Library Models, Genetic Molecular Sequence Data NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Oncogene Protein v-maf Oncogene Proteins, Viral/genetics,metabolism Protein Binding Protein Conformation Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-fos/metabolism Proto-Oncogene Proteins c-jun/metabolism Proto-Oncogene Proteins c-maf Repressor Proteins/genetics,metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Suppression, Genetic Transcription Factors/metabolism Viral Proteins
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Oncogene Protein v-maf Oncogene Proteins, Viral Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Proto-Oncogene Proteins c-maf Repressor Proteins Transcription Factors Viral Proteins v-Maf protein, Avian retrovirus AS42
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kataoka K
Department of Viral Oncology, Cancer Institute, Tokyo, Japan.
Igarashi K
Itoh K
Fujiwara K T
Noda M
Yamamoto M
Nishizawa M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.4.2180
Published
1995-04-00
Pages
2180-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230446
Subset
IM
Databases
GENBANK
D28601, D28602
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/MCB.15.4.2180 PMC Full Text View journal details
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