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PMID: 7751295 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Physical and genetic map of the Lactococcus lactis subsp. cremoris MG1363 chromosome: comparison with that of Lactococcus lactis subsp. lactis IL 1403 reveals a large genome inversion.

Journal of bacteriology ·Vol. 177 ·No. 10 ·1995-05-00 ·Pages 2840-50

Le Bourgeois P, Lautier M, van den Berghe L, Gasson MJ, Ritzenthaler P

Abstract

A physical and genetic map of the chromosome of the Lactococcus lactis subsp. cremoris reference strain MG1363 was established. The physical map was constructed for NotI, ApaI, and SmaI enzymes by using a strategy that combines creation of new rare restriction sites by the random-integration vector pRL1 and ordering of restriction fragments by indirect end-labeling experiments. The MG1363 chromosome appeared to be circular and 2,560 kb long. Seventy-seven chromosomal markers were located on the physical map by hybridization experiments. Integration via homologous recombination of pRC1-derived plasmids allowed a more precise location of some lactococcal genes and determination of their orientation on the chromosome. The MG1363 chromosome contains six rRNA operons; five are clustered within 15% of the chromosome and transcribed in the same direction. Comparison of the L. lactis subsp. cremoris MG1363 physical map with those of the two L. lactis subsp. lactis strains IL1403 and DL11 revealed a high degree of restriction polymorphism. At the genetic organization level, despite an overall conservation of gene organization, strain MG1363 presents a large inversion of half of the genome in the region containing the rRNA operons.

MeSH Terms
Bacteriophages/genetics Chromosome Mapping Chromosomes, Bacterial/genetics DNA, Bacterial/genetics,metabolism DNA, Ribosomal/genetics Deoxyribonucleases, Type II Site-Specific/metabolism Genetic Markers Lactococcus lactis/genetics Mutagenesis, Insertional Nucleic Acid Hybridization Operon/genetics Restriction Mapping Species Specificity
Chemicals
DNA, Bacterial DNA, Ribosomal Genetic Markers CCCGGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific GCGGCCGC-specific type II deoxyribonucleases GGGCCC-specific type II deoxyribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Le Bourgeois P
Laboratoire de Microbiologie et Génétique Moléculaire, Institut de Biologie Cellulaire et de Génétique du Centre National de la Recherche Scientifique, Toulouse, France.
Lautier M
van den Berghe L
Gasson M J
Ritzenthaler P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.10.2840-2850.1995
Published
1995-05-00
Pages
2840-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176957
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.10.2840-2850.1995 PMC Full Text View journal details
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