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PMID: 9315642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alleviation of histone H1-mediated transcriptional repression and chromatin compaction by the acidic activation region in chromosomal protein HMG-14.

Molecular and cellular biology ·Vol. 17 ·No. 10 ·1997-10-00 ·Pages 5843-55

Ding HF, Bustin M, Hansen U

Abstract

Histone H1 promotes the generation of a condensed, transcriptionally inactive, higher-order chromatin structure. Consequently, histone H1 activity must be antagonized in order to convert chromatin to a transcriptionally competent, more extended structure. Using simian virus 40 minichromosomes as a model system, we now demonstrate that the nonhistone chromosomal protein HMG-14, which is known to preferentially associate with active chromatin, completely alleviates histone H1-mediated inhibition of transcription by RNA polymerase II. HMG-14 also partially disrupts histone H1-dependent compaction of chromatin. Both the transcriptional enhancement and chromatin-unfolding activities of HMG-14 are mediated through its acidic, C-terminal region. Strikingly, transcriptional and structural activities of HMG-14 are maintained upon replacement of the C-terminal fragment by acidic regions from either GAL4 or HMG-2. These data support the model that the acidic C terminus of HMG-14 is involved in unfolding higher-order chromatin structure to facilitate transcriptional activation of mammalian genes.

MeSH Terms
Amino Acid Sequence Chromatin/metabolism HeLa Cells High Mobility Group Proteins/chemistry,genetics,physiology Histones/metabolism Humans Models, Genetic Molecular Sequence Data RNA Polymerase II/metabolism RNA, Viral/biosynthesis Recombinant Fusion Proteins Sequence Deletion Simian virus 40/genetics Transcription, Genetic/physiology Transcriptional Activation/physiology
Chemicals
Chromatin High Mobility Group Proteins Histones RNA, Viral Recombinant Fusion Proteins RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ding H F
Dana-Farber Cancer Institute, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Bustin M
Hansen U
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.10.5843
Published
1997-10-00
Pages
5843-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232432
Subset
IM
Grants
NCI NIH HHS · 5T32CA0936 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.10.5843 PMC Full Text View journal details
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