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PMID: 9725903 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Partially processed pre-rRNA is preserved in association with processing components in nucleolus-derived foci during mitosis.

Molecular biology of the cell ·Vol. 9 ·No. 9 ·1998-09-00 ·Pages 2407-22

Dundr M, Olson MO

Abstract

Previous studies showed that components implicated in pre-rRNA processing, including U3 small nucleolar (sno)RNA, fibrillarin, nucleolin, and proteins B23 and p52, accumulate in perichromosomal regions and in numerous mitotic cytoplasmic particles, termed nucleolus-derived foci (NDF) between early anaphase and late telophase. The latter structures were analyzed for the presence of pre-rRNA by fluorescence in situ hybridization using probes for segments of pre-rRNA with known half-lives. The NDF did not contain the short-lived 5'-external transcribed spacer (ETS) leader segment upstream from the primary processing site in 47S pre-rRNA. However, the NDF contained sequences from the 5'-ETS core, 18S, internal transcribed spacer 1 (ITS1), and 28S segments and also had detectable, but significantly reduced, levels of the 3'-ETS sequence. Northern analyses showed that in mitotic cells, the latter sequences were present predominantly in 45S-46S pre-rRNAs, indicating that high-molecular weight processing intermediates are preserved during mitosis. Two additional essential processing components were also found in the NDF: U8 snoRNA and hPop1 (a protein component of RNase MRP and RNase P). Thus, the NDF appear to be large complexes containing partially processed pre-rRNA associated with processing components in which processing has been significantly suppressed. The NDF may facilitate coordinated assembly of postmitotic nucleoli.

MeSH Terms
5' Untranslated Regions Animals Apoptosis Regulatory Proteins Carrier Proteins Cell Line Cell Nucleolus/metabolism Endoribonucleases/metabolism Haplorhini Humans Mitosis/physiology RNA Precursors RNA Processing, Post-Transcriptional RNA, Catalytic/metabolism RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Small Nuclear Ribonuclease P Ribonucleoproteins/analysis
Chemicals
5' Untranslated Regions Apoptosis Regulatory Proteins Carrier Proteins POP1 protein, human RNA Precursors RNA, Catalytic RNA, Ribosomal, 18S RNA, Ribosomal, 28S RNA, Small Nuclear Ribonucleoproteins Endoribonucleases mitochondrial RNA-processing endoribonuclease RPP14 protein, human Ribonuclease P
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dundr M
Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA.
Olson M O
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.9.9.2407
Published
1998-09-00
Pages
2407-22
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25507
Subset
IM
Grants
NIAID NIH HHS · AI-34277 · United States
NIGMS NIH HHS · GM-28349 · United States
External Links
PubMed source DOI: 10.1091/mbc.9.9.2407 PMC Full Text View journal details
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