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PMID: 15075271 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Challenge of Drosophila melanogaster with Cryptococcus neoformans and role of the innate immune response.

Eukaryotic cell ·Vol. 3 ·No. 2 ·2004-04-00 ·Pages 413-9

Apidianakis Y, Rahme LG, Heitman J, Ausubel FM, Calderwood SB, Mylonakis E

Abstract

We found that the ingestion of Cryptococcus neoformans by Drosophila melanogaster resulted in the death of the fly but that the ingestion of Saccharomyces cerevisiae or the nonpathogenic Cryptococcus kuetzingii or Cryptococcus laurentii did not. The C. neoformans protein kinase A and RAS signal transduction pathways, previously shown to be involved in virulence in mammals, also played a role in killing Drosophila. Mutation of the Toll immune response pathway, the predominant antifungal pathway of the fly, did not play a role in Drosophila defense following ingestion of the yeast. However, the Toll pathway was necessary for the clearance of C. neoformans introduced directly into the hemolymph of D. melanogaster and for the survival of systemically infected flies.

MeSH Terms
Animals Colony Count, Microbial Cryptococcus neoformans/genetics,pathogenicity Cyclic AMP-Dependent Protein Kinases Digestive System/immunology,microbiology Drosophila Proteins/immunology,metabolism Drosophila melanogaster/genetics,immunology,microbiology Immunity, Innate Mutation Protein Serine-Threonine Kinases/immunology,metabolism Saccharomyces cerevisiae/genetics,immunology Signal Transduction Virulence Factors/immunology ras Proteins/immunology,metabolism
Chemicals
Drosophila Proteins Virulence Factors imd protein, Drosophila spz protein, Drosophila Protein Serine-Threonine Kinases Cyclic AMP-Dependent Protein Kinases Ras85D protein, Drosophila ras Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Apidianakis Yiorgos
Department of Surgery, Massachusetts General Hospital. Shriner's Burns Institute, Boston, Massachusetts 02114, USA.
Rahme Laurence G
Heitman Joseph
Ausubel Frederick M
Calderwood Stephen B
Mylonakis Eleftherios
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.3.2.413-419.2004
Published
2004-04-00
Pages
413-9
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC387633
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048707 · United States
NIGMS NIH HHS · R37 GM048707 · United States
NIGMS NIH HHS · GM48707 · United States
External Links
PubMed source DOI: 10.1128/EC.3.2.413-419.2004 PMC Full Text View journal details
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