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PMID: 2014170 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

Nucleic acids research ·Vol. 19 ·No. 2 ·1991-01-25 ·Pages 307-11

Stefan C, Xia YN, Van Etten JL

Abstract

The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.

Related Genes
M.CviRI
MeSH Terms
Amino Acid Sequence Base Sequence Chlorella Cloning, Molecular DNA Modification Methylases/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization Plasmids Sequence Alignment Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics Viruses/enzymology
Chemicals
DNA Modification Methylases DNA modification methylase CviRI Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stefan C
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Xia Y N
Van Etten J L
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.2.307
Published
1991-01-25
Pages
307-11
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333595
Subset
IM
Grants
NIGMS NIH HHS · GM-32441 · United States
Databases
GENBANK
M38173, X56859, X56860, X56861, X56862, X56863, X56864, X56865, X57509, X57510
External Links
PubMed source DOI: 10.1093/nar/19.2.307 PMC Full Text View journal details
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