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PMID: 2164635 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lipopolysaccharide and dexamethasone induce mouse mammary tumor proviral gene expression and differentiation in B lymphocytes through distinct regulatory pathways.

Molecular and cellular biology ·Vol. 10 ·No. 8 ·1990-08-00 ·Pages 4211-20

King LB, Corley RB

Abstract

Endogenous mouse mammary tumor virus (MMTV) proviral transcripts are up regulated during the normal course of B-lymphocyte differentiation. We report here that the regulatory mechanisms which lead to increased levels of MMTV transcripts in differentiating, lipopolysaccharide (LPS)-stimulated normal B cells and in the inducible B-cell lymphoma line CH12 are at least partially distinct from those controlling increases in immunoglobulin and J-chain gene expression. In studies designed to characterize the stimulatory pathways leading to MMTV expression in CH12 cells, we found that stimulation with either LPS or dexamethasone (Dex), a transcriptional activator of MMTV genes, induced not only MMTV expression but also differentiation to antibody secretion. Only Dex-induced and not LPS-induced MMTV expression and differentiation were inhibited by the glucocorticoid antagonist RU486, demonstrating that Dex and LPS stimulate B cells by distinct molecular pathways. Therefore, in B cells, MMTV expression can be regulated via either the conventional hormone receptor-dependent pathway or a hormone receptor-independent pathway. Furthermore, these results suggest that steroid stimulation of B cells can lead to alterations in the expression of other results suggest that steroid stimulation of B cells can lead to alterations in the expression of other steroid-responsive genes that can become involved in the process of B-cell differentiation.

MeSH Terms
Animals B-Lymphocytes/cytology,drug effects,immunology Cell Differentiation/drug effects Cell Line Cell Transformation, Viral Dexamethasone/pharmacology Gene Expression/drug effects Gene Expression Regulation, Viral Genes, Immunoglobulin/drug effects Immunoglobulin mu-Chains/genetics Kinetics Lipopolysaccharides/pharmacology Mammary Tumor Virus, Mouse/drug effects,genetics Mice Mifepristone/pharmacology Proviruses/drug effects,genetics Transcription, Genetic/drug effects
Chemicals
Immunoglobulin mu-Chains Lipopolysaccharides Mifepristone Dexamethasone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
King L B
Department of Microbiology and Immunology, Duke Medical Center, Durham, North Carolina 27710.
Corley R B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.8.4211-4220.1990
Published
1990-08-00
Pages
4211-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360955
Subset
IM
Grants
NCI NIH HHS · CA36642 · United States
NCI NIH HHS · T32 CA09058 · United States
NIGMS NIH HHS · T32 GM07184 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.8.4211-4220.1990 PMC Full Text View journal details
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