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PMID: 25815584 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Juxtaposition of heterozygous and homozygous regions causes reciprocal crossover remodelling via interference during Arabidopsis meiosis.

eLife ·Vol. 4 ·2015-03-27

Ziolkowski PA, Berchowitz LE, Lambing C, Yelina NE, Zhao X, Kelly KA, Choi K, Ziolkowska L, June V, Sanchez-Moran E, Franklin C, Copenhaver GP, Henderson IR

Abstract

During meiosis homologous chromosomes undergo crossover recombination. Sequence differences between homologs can locally inhibit crossovers. Despite this, nucleotide diversity and population-scaled recombination are positively correlated in eukaryote genomes. To investigate interactions between heterozygosity and recombination we crossed Arabidopsis lines carrying fluorescent crossover reporters to 32 diverse accessions and observed hybrids with significantly higher and lower crossovers than homozygotes. Using recombinant populations derived from these crosses we observed that heterozygous regions increase crossovers when juxtaposed with homozygous regions, which reciprocally decrease. Total crossovers measured by chiasmata were unchanged when heterozygosity was varied, consistent with homeostatic control. We tested the effects of heterozygosity in mutants where the balance of interfering and non-interfering crossover repair is altered. Crossover remodeling at homozygosity-heterozygosity junctions requires interference, and non-interfering repair is inefficient in heterozygous regions. As a consequence, heterozygous regions show stronger crossover interference. Our findings reveal how varying homolog polymorphism patterns can shape meiotic recombination.

Keywords
arabidopsis chromosomes crossover evolutionary biology genes genomics interference meiosis natural variation recombination
MeSH Terms
Arabidopsis/cytology,genetics Chromosomes, Plant/genetics Crosses, Genetic Crossing Over, Genetic Ecotype Fluorescence Genetic Variation Genotype Heterozygote Homozygote Meiosis/genetics
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Ziolkowski Piotr A
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Berchowitz Luke E
Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, United States.
Lambing Christophe
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Yelina Nataliya E
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Zhao Xiaohui
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Kelly Krystyna A
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Choi Kyuha
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Ziolkowska Liliana
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
June Viviana
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
Sanchez-Moran Eugenio
School of Biosciences, University of Birmingham, Birmingham, United Kingdom.
Franklin Chris
School of Biosciences, University of Birmingham, Birmingham, United Kingdom.
Copenhaver Gregory P ORCID
Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, United States.
Henderson Ian R
Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.
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Article Info
Journal
eLife
Abbr.
Elife
ISSN
2050-084X
DOI
10.7554/eLife.03708
Published
2015-03-27
Epub
2015-00-27
Language
English
Region
England
NLM ID
101579614
PMCID
PMC4407271
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/K007882/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/M004937/1 · United Kingdom
External Links
PubMed source DOI: 10.7554/eLife.03708 PMC Full Text View journal details
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