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PMID: 2731964 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics and expression of the Q6 and Q8 genes. An LTR-like sequence in the 3' untranslated region.

Immunogenetics ·Vol. 29 ·No. 6 ·1989-00-00 ·Pages 371-9

Elliott E, Rathbun D, Ramsingh A, Garberi J, Flaherty L

Abstract

A unique 2.2-kb mRNA is transcribed from the Q6 and Q8 genes of the mouse major histocompatibility complex. RNase protection experiments and DNA sequence analysis have mapped the 3' terminus to a site located 1110 bp downstream from exon 8. Comparison of the 3' sequence of Q8 to that of Q7 revealed that the two genes diverge from one another at a point located 200 bp into the untranslated domain. This finding explains the increased size of the transcript. RNase protection experiments involving twelve different strains of mice have revealed few sequence polymorphisms. A tissue distribution of the 2.2-kb transcript in B6 mice revealed that the highest quantities of message were present in the spleen with decreasingly lower amounts in the thymus, liver, kidney, testis, and brain. The H-2r haplotype appeared novel; it is phenotypically Qa-2-, yet expressed a 2.2-kb transcript which hybridizes to a Q8 probe. A comparison of a Qa-2hi expressor, B6, and a Qa-2low expressor, BALB/cFla, has established that these quantitative cell surface differences are reflected in mRNA differences. A homology search of the Genbank database has revealed that the 3' portion of Q8 contains extensive homology to a retrovirus-like long terminal repeat sequence that is characteristic of an embryonic-specific transposon (ETn).

MeSH Terms
Alleles Animals Base Sequence Genes, MHC Class I Genetic Variation Histocompatibility Antigens Class I/genetics Mice Mice, Inbred BALB C Mice, Inbred DBA Molecular Sequence Data Nucleotide Mapping Phenotype Repetitive Sequences, Nucleic Acid Tissue Distribution Transcription, Genetic
Chemicals
Histocompatibility Antigens Class I Q surface antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Elliott E
Department of Microbiology and Immunology, Albany Medical College, New York.
Rathbun D
Ramsingh A
Garberi J
Flaherty L
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00375865
Published
1989-00-00
Pages
371-9
Language
English
Region
United States
NLM ID
0420404
Subset
IM
Grants
NIGMS NIH HHS · GM 37947 · United States
Databases
GENBANK
M22733
External Links
PubMed source DOI: 10.1007/BF00375865 View journal details
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