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PMID: 2988935 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The human ubiquitin multigene family: some genes contain multiple directly repeated ubiquitin coding sequences.

The EMBO journal ·Vol. 4 ·No. 3 ·1985-03-00 ·Pages 755-9

Wiborg O, Pedersen MS, Wind A, Berglund LE, Marcker KA, Vuust J

Abstract

Ubiquitin coding sequences were isolated from a human genomic library and two cDNA libraries. One human ubiquitin gene consists of 2055 nucleotides and codes for a polyprotein consisting of 685 amino acid residues. The polyprotein contains nine direct repeats of the ubiquitin amino acid sequence and the last ubiquitin sequence is extended with an additional valyl residue at the C-terminal end. No spacer sequences separate the ubiquitin repeats and the coding regions are not interrupted by intervening sequences. This particular gene is transcribed since cDNAs corresponding to the genomic sequence have been isolated. At least two more types of ubiquitin genes are encoded in the human genome, one coding for an ubiquitin monomer while another presumably codes for three or four direct repeats of the ubiquitin sequence. Human DNA contains many copies of the ubiquitin sequence. Ubiquitin is therefore encoded in the human genome as a multigene family.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Genes High Mobility Group Proteins/genetics Humans Mice Molecular Weight Repetitive Sequences, Nucleic Acid Swine Ubiquitins/genetics
Chemicals
High Mobility Group Proteins Ubiquitins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wiborg O
Pedersen M S
Wind A
Berglund L E
Marcker K A
Vuust J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-03-00
Pages
755-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554252
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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