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PMID: 9418908 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pex19p, a farnesylated protein essential for peroxisome biogenesis.

Molecular and cellular biology ·Vol. 18 ·No. 1 ·1998-01-00 ·Pages 616-28

Götte K, Girzalsky W, Linkert M, Baumgart E, Kammerer S, Kunau WH, Erdmann R

Abstract

We report the identification and molecular characterization of Pex19p, an oleic acid-inducible, farnesylated protein of 39.7 kDa that is essential for peroxisome biogenesis in Saccharomyces cerevisiae. Cells lacking Pex19p are characterized by the absence of morphologically detectable peroxisomes and mislocalization of peroxisomal matrix proteins to the cytosol. The human HK33 gene product was identified as the putative human ortholog of Pex19p. Evidence is provided that farnesylation of Pex19p takes place at the cysteine of the C-terminal CKQQ amino acid sequence. Farnesylation of Pex19p was shown to be essential for the proper function of the protein in peroxisome biogenesis. Pex19p was shown to interact with Pex3p in vivo, and this interaction required farnesylation of Pex19p.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Fungal Proteins/physiology Gene Expression Regulation, Fungal Humans Membrane Proteins/genetics Microbodies/physiology Molecular Sequence Data PHEX Phosphate Regulating Neutral Endopeptidase Proteins/genetics Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Alignment
Chemicals
Fungal Proteins Membrane Proteins PEX19 protein, S cerevisiae Proteins Saccharomyces cerevisiae Proteins PHEX Phosphate Regulating Neutral Endopeptidase PHEX protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Götte K
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Germany.
Girzalsky W
Linkert M
Baumgart E
Kammerer S
Kunau W H
Erdmann R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.1.616
Published
1998-01-00
Pages
616-28
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC121529
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.18.1.616 PMC Full Text View journal details
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