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PMID: 9560370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.

Genetics ·Vol. 148 ·No. 4 ·1998-04-00 ·Pages 1507-24

Lobachev KS, Shor BM, Tran HT, Taylor W, Keen JD, Resnick MA, Gordenin DA

Abstract

Inverted DNA repeats are an at-risk motif for genetic instability that can induce both deletions and recombination in yeast. We investigated the role of the length of inverted repeats and size of the DNA separating the repeats for deletion and recombination. Stimulation of both deletion and recombination was directly related to the size of inverted repeats and inversely related to the size of intervening spacers. A perfect palindrome, formed by two 1.0-kb URA3-inverted repeats, increased intra- and interchromosomal recombination in the adjacent region 2,400-fold and 17,000-fold, respectively. The presence of a strong origin of replication in the spacer reduced both rates of deletion and recombination. These results support a model in which the stimulation of deletion and recombination by inverted repeats is initiated by a secondary structure formed between single-stranded DNA of inverted repeats during replication.

MeSH Terms
Chromosomes, Fungal Crossing Over, Genetic DNA, Fungal Recombination, Genetic Repetitive Sequences, Nucleic Acid Replication Origin Saccharomyces cerevisiae/genetics Sequence Deletion
Chemicals
DNA, Fungal
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lobachev K S
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
Shor B M
Tran H T
Taylor W
Keen J D
Resnick M A
Gordenin D A
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/148.4.1507
Published
1998-04-00
Pages
1507-24
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460095
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/148.4.1507 PMC Full Text View journal details
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