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PMID: 1729599 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mechanism of translation of monocistronic and multicistronic human immunodeficiency virus type 1 mRNAs.

Molecular and cellular biology ·Vol. 12 ·No. 1 ·1992-01-00 ·Pages 207-19

Schwartz S, Felber BK, Pavlakis GN

Abstract

We have used a panel of cDNA clones expressing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) mRNAs to study translation of these mRNAs in eucaryotic cells. The tat open reading frame (ORF) has a strong signal for translation initiation, while rev and vpu ORFs have weaker signals. The expression of downstream ORFs is inhibited in mRNAs that contain the tat ORF as the first ORF. In contrast, downstream ORFs are expressed efficiently from mRNAs that have rev or vpu as the first ORF. All env mRNAs contain the upstream vpu ORF. Expression of HIV-1 Env protein requires a weak vpu AUG, which allows leaky scanning to occur, thereby allowing ribosomes access to the downstream env ORF. We concluded that HIV-1 mRNAs are translated by the scanning mechanism and that expression of more than one protein from each mRNA was caused by leaky scanning at the first AUG of the mRNA.

Related Genes
apu rev tat
MeSH Terms
Base Sequence DNA, Viral Gene Expression Regulation, Viral Genes, rev Genes, tat HIV-1/genetics Molecular Sequence Data Mutation Open Reading Frames Precipitin Tests Protein Biosynthesis RNA Splicing RNA, Messenger/metabolism RNA, Viral/metabolism
Chemicals
DNA, Viral RNA, Messenger RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartz S
Human Retrovirus Section, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Felber B K
Pavlakis G N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.1.207-219.1992
Published
1992-01-00
Pages
207-19
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364085
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
K03455
External Links
PubMed source DOI: 10.1128/mcb.12.1.207-219.1992 PMC Full Text View journal details
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