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PMID: 17368442 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural

Transcriptome profiling of the C. elegans Rb ortholog reveals diverse developmental roles.

Developmental biology ·Vol. 305 ·No. 2 ·2007-05-15 ·Pages 674-84

Kirienko NV, Fay DS

Abstract

LIN-35 is the single C. elegans ortholog of the mammalian pocket protein family members, pRb, p107, and p130. To gain insight into the roles of pocket proteins during development, a microarray analysis was performed with lin-35 mutants. Stage-specific regulation patterns were revealed, indicating that LIN-35 plays diverse roles at distinct developmental stages. LIN-35 was found to repress the expression of many genes involved in cell proliferation in larvae, an activity that is carried out in conjunction with E2F. In addition, LIN-35 was found to regulate neuronal genes during embryogenesis and targets of the intestinal-specific GATA transcription factor, ELT-2, at multiple developmental stages. Additional findings suggest that LIN-35 functions in cell cycle regulation in embryos in a manner that is independent of E2F. A comparison of LIN-35-regulated genes with known fly and mammalian pocket protein targets revealed a high degree of overlap, indicating strong conservation of pocket protein functions in diverse phyla. Based on microarray results and our refinement of the C. elegans E2F consensus sequence, we were able to generate a comprehensive list of putative E2F-regulated genes in C. elegans. These results implicate a large number of genes previously unconnected to cell cycle control as having potential roles in this process.

MeSH Terms
Amino Acid Motifs/genetics Animals Caenorhabditis elegans/embryology,genetics,metabolism Caenorhabditis elegans Proteins/biosynthesis,genetics E2F Transcription Factors/biosynthesis,genetics,physiology Gene Expression Profiling Gene Expression Regulation, Developmental/physiology Oligonucleotide Array Sequence Analysis Repressor Proteins/biosynthesis,genetics Retinoblastoma Protein/biosynthesis,genetics,physiology
Chemicals
Caenorhabditis elegans Proteins E2F Transcription Factors Repressor Proteins Retinoblastoma Protein lin-35 protein, C elegans
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirienko Natalia V
Department of Molecular Biology, College of Agriculture, University of Wyoming, Dept. 3944, 1000 E. University Avenue, Laramie, WY 82071, USA.
Fay David S
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Article Info
Journal
Developmental biology
Abbr.
Dev Biol
ISSN
0012-1606
DOI
10.1016/j.ydbio.2007.02.021
Published
2007-05-15
Epub
2007-00-21
Pages
674-84
Language
English
Region
United States
NLM ID
0372762
PMCID
PMC2680605
Subset
IM
Grants
NIGMS NIH HHS · R01 GM066868 · United States
NIGMS NIH HHS · R01 GM066868-01A1 · United States
External Links
PubMed source DOI: 10.1016/j.ydbio.2007.02.021 PMC Full Text View journal details
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