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PMID: 20427571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Peroxisomal membrane proteins insert into the endoplasmic reticulum.

Molecular biology of the cell ·Vol. 21 ·No. 12 ·2010-06-15 ·Pages 2057-65

van der Zand A, Braakman I, Tabak HF

Abstract

We show that a comprehensive set of 16 peroxisomal membrane proteins (PMPs) encompassing all types of membrane topologies first target to the endoplasmic reticulum (ER) in Saccharomyces cerevisiae. These PMPs insert into the ER membrane via the protein import complexes Sec61p and Get3p (for tail-anchored proteins). This trafficking pathway is representative for multiplying wild-type cells in which the peroxisome population needs to be maintained, as well as for mutant cells lacking peroxisomes in which new peroxisomes form after complementation with the wild-type version of the mutant gene. PMPs leave the ER in a Pex3p-Pex19p-dependent manner to end up in metabolically active peroxisomes. These results further extend the new concept that peroxisomes derive their basic framework (membrane and membrane proteins) from the ER and imply a new functional role for Pex3p and Pex19p.

MeSH Terms
Endoplasmic Reticulum/metabolism Intracellular Membranes/metabolism Membrane Proteins/chemistry,metabolism Peroxisomes/metabolism Protein Transport Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Membrane Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van der Zand Adabella
Cellular Protein Chemistry, Faculty of Science, Utrecht University, NL-3584 CH Utrecht, The Netherlands.
Braakman Ineke
Tabak Henk F
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e10-02-0082
Published
2010-06-15
Epub
2010-00-28
Pages
2057-65
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2883949
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e10-02-0082 PMC Full Text View journal details
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