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PMID: 8670884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular basis of two subfamilies of immunoglobulin-like chaperones.

The EMBO journal ·Vol. 15 ·No. 15 ·1996-08-01 ·Pages 3792-805

Hung DL, Knight SD, Woods RM, Pinkner JS, Hultgren SJ

Abstract

The initial encounter of a microbial pathogen with the host often involves the recognition of host receptors by different kinds of bacterial adhesive organelles called pili, fimbriae, fibrillae or afimbrial adhesins. The development of over 26 of these architecturally diverse adhesive organelles in various Gram-negative pathogens depends on periplasmic chaperones that are comprised of two immunoglobulin-like domains. All of the chaperones possess a highly conserved sheet in domain 1 and a conserved interdomain hydrogen-bonding network. Chaperone-subunit complex formation depends on the anchoring of the carboxylate group of the subunit into the conserved crevice of the chaperone cleft and the subsequent positioning of the COOH terminus of subunits along the exposed edge of the conserved sheet of the chaperone. We discovered that the chaperones can be divided into two distinct subfamilies based upon conserved structural differences that occur in the conserved sheet. Interestingly, a subdivision of the chaperones based upon whether they assemble rod-like pili or non-pilus organelles that have an atypical morphology defines the same two subgroups. The molecular dissection of the two chaperone subfamilies and the adhesive fibers that they assemble has advanced our understanding of the development of virulence-associated organelles in pathogenic bacteria.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,classification Conserved Sequence Crystallography, X-Ray Enzyme-Linked Immunosorbent Assay Models, Molecular Molecular Chaperones/chemistry,classification Molecular Sequence Data Protein Conformation
Chemicals
Bacterial Proteins Molecular Chaperones
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hung D L
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA.
Knight S D
Woods R M
Pinkner J S
Hultgren S J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-08-01
Pages
3792-805
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452060
Subset
IM
Grants
NIAID NIH HHS · AI07172-16 · United States
NIAID NIH HHS · R01AI29549 · United States
External Links
PubMed source PMC Full Text View journal details
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