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PMID: 32120839 Published · epublish English Journal Article Review

Proteomic Analyses of Human Sperm Cells: Understanding the Role of Proteins and Molecular Pathways Affecting Male Reproductive Health.

International journal of molecular sciences ·Vol. 21 ·No. 5 ·2020-02-27

Agarwal A, Panner Selvam MK, Baskaran S

Abstract

Human sperm proteomics research has gained increasing attention lately, which provides complete information about the functional state of the spermatozoa. Changes in the sperm proteome are evident in several male infertility associated conditions. Global proteomic tools, such as liquid chromatography tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight, are used to profile the sperm proteins to identify the molecular pathways that are defective in infertile men. This review discusses the use of proteomic techniques to analyze the spermatozoa proteome. It also highlights the general steps involved in global proteomic approaches including bioinformatic analysis of the sperm proteomic data. Also, we have presented the findings of major proteomic studies and possible biomarkers in the diagnosis and therapeutics of male infertility. Extensive research on sperm proteome will help in understanding the role of fertility associated sperm proteins. Validation of the sperm proteins as biomarkers in different male infertility conditions may aid the physician in better clinical management.

Keywords
bioinformatics male infertility molecular pathways proteomics sperm
MeSH Terms
Asthenozoospermia/metabolism Azoospermia/metabolism Biomarkers/metabolism Chromatography, Liquid Computational Biology/methods Humans Infertility, Male/metabolism Male Proteomics/methods Signal Transduction/genetics Spermatozoa/metabolism Tandem Mass Spectrometry Teratozoospermia/metabolism Testicular Neoplasms/metabolism Varicocele/metabolism
Chemicals
Biomarkers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Agarwal Ashok ORCID
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
Panner Selvam Manesh Kumar
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
Baskaran Saradha
American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH 44195, USA.
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Article Info
Journal
International journal of molecular sciences
Abbr.
Int J Mol Sci
ISSN
1422-0067
DOI
10.3390/ijms21051621
Published
2020-02-27
Epub
2020-00-27
Language
English
Region
Switzerland
NLM ID
101092791
PMCID
PMC7084638
Subset
IM
External Links
PubMed source DOI: 10.3390/ijms21051621 PMC Full Text View journal details
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