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PMID: 8887638 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Bach proteins belong to a novel family of BTB-basic leucine zipper transcription factors that interact with MafK and regulate transcription through the NF-E2 site.

Molecular and cellular biology ·Vol. 16 ·No. 11 ·1996-11-00 ·Pages 6083-95

Oyake T, Itoh K, Motohashi H, Hayashi N, Hoshino H, Nishizawa M, Yamamoto M, Igarashi K

Abstract

Members of the small Maf family (MafK, MafF, and MafG) are basic region leucine zipper (bZip) proteins that can function as transcriptional activators or repressors. The dimer compositions of their DNA binding forms determine whether the small Maf family proteins activate or repress transcription. Using a yeast two-hybrid screen with a GAL4-MafK fusion protein, we have identified two novel bZip transcription factors, Bach1 and Bach2, as heterodimerization partners of MafK. In addition to a Cap'n'collar-type bZip domain, these Bach proteins possess a BTB domain which is a protein interaction motif; Bach1 and Bach2 show significant similarity to each other in these regions but are otherwise divergent. Whereas expression of Bach1 appears ubiquitous, that of Bach2 is restricted to monocytes and neuronal cells. Bach proteins bind in vitro to NF-E2 binding sites, recognition elements for the hematopoietic transcription factor NF-E2, by forming heterodimers with MafK. Furthermore, a DNA binding complex that contained MafK as well as Bach2 or a protein related closely to Bach2 was found to be present in mouse brain cells. Bach1 and Bach2 function as transcription repressors in transfection assays using fibroblast cells, but they function as a transcriptional activator and repressor, respectively, in cultured erythroid cells. The results suggest that members of the Bach family play important roles in coordinating transcription activation and repression by MafK.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Basic-Leucine Zipper Transcription Factors Binding Sites Brain/metabolism Cloning, Molecular Consensus Sequence DNA-Binding Proteins/metabolism Dimerization Erythroid-Specific DNA-Binding Factors Leucine Zippers MafK Transcription Factor Mice Molecular Sequence Data Monocytes/metabolism NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Neurons/metabolism Nuclear Proteins/chemistry,metabolism Recombinant Fusion Proteins/chemistry,metabolism Saccharomyces cerevisiae Sequence Homology, Amino Acid Transcription Factors/chemistry,metabolism Transcription, Genetic Zinc Fingers
Chemicals
BACH2 protein, human Bach1 protein, mouse Bach2 protein, mouse Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors MafK Transcription Factor Mafk protein, mouse NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Nfe2 protein, mouse Nuclear Proteins Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Oyake T
Department of Biochemistry, Tohoku University School of Medicine, Sendai, Japan.
Itoh K
Motohashi H
Hayashi N
Hoshino H
Nishizawa M
Yamamoto M
Igarashi K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.11.6083
Published
1996-11-00
Pages
6083-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231611
Subset
IM
Databases
GENBANK
D86603, D86604
External Links
PubMed source DOI: 10.1128/MCB.16.11.6083 PMC Full Text View journal details
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